Q-STAIN X (QSXTM)-Dir Pan-CK 4Abs (Clone AE1, AE3, C94, R226) and Q-STAIN X (QSXTM)-Enh Pan-CK 4Abs (Clone AE1, AE3, C94, R226) both are intended for laboratory use to qualitatively identify by light microscopy the presence of Pan-CK protein in sections of formalin-fixed, paraffinembedded (FFPE) and/or fresh frozen tissues using immunohistochemistry (IHC) test methods. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests and proper controls interpreted by a qualified pathologist and/or physician. This reagent has been formulated to ready-to-use concentration and optimized for IHC use without further dilution.
Summary and Explanation:
QSX (-Dir and -Enh) Pan-CK 4Abs is ready-to-use antibody cocktail which consists of equal amounts of three mouse monoclonal antibodies clones AE1, AE3 and C94 and one rabbit monoclonal antibody clone R226. Clones AE1 and AE3, always used together as an anti-pan-cytokeratin antibody cocktail, recognize cytokeratin 1-8, 10, 14 -16 and 19; clone C94 recognizes cytokeratin 8 and 18; clone R226 specifically recognizes CK5. The classic
Pan-CK antibody cocktail AE1/AE3 has been the most popular Pan-CK cocktail in the market and is known to have limited reactivity in hepatocellular carcinomas (HCCs), renal cell carcinomas (RCCs), and pulmonary small cell carcinomas, and even on FFPE tissue specimens. See Q-STAIN X User’s Guide to learn more about test protocols.
