Q-STAIN (QSXTM)-Enh CK8/18 (anti-human cytokeratin 8/18) antibody is intended for laboratory use to qualitatively identify by light microscopy the presence of CK8/18 in sections of formalin-fixed, paraffin-embedded tissue sections or frozen tissues using immunohistochemistry (IHC) test methods. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist/physician. These reagents have been formulated to ready-to-use concentration and optimized for IHC on specific tissue application use without further dilution.
Summary and Explanation:
QSX-Enh CK8/18 is a ready-to-use mouse monoclonal antibody reagent (clone C94). The CK8/18 antibody recognizes human cytokeratin intermediate low molecular weight filament proteins of 52.5 kD and 45 kD, i.e., cytokeratins 8 and 18, respectively. Cytokeratins 8 and 18 are expressed in most simple and glandular epithelium, such as thyroid, female breast, gastrointestinal tract, and respiratory tract. In cancer tissues, adenocarcinomas express these proteins, but keratinizing squamous cell carcinomas do not. Antibodies to CK8 and CK18 may be utilized in the classification of tumors of epithelial origin.1,2 See Q-STAIN X User’s Guide to learn more about test protocols.
